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KMID : 0043319980210030260
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Archives of Pharmacal Research
1998 Volume.21 No. 3 p.260 ~ p.265
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In vitro Angiogenic Activity of Aloe vera Gel on Calf Pulmonary Artery Endothelial (CPAE) Cells
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Lee Myoung-Jin
Lee Ok-Hee
Yoon Soo-Hong
Lee Seung-Ki
Chung Myung-Hee
Park Young-In
Sung Chung-Ki
Choi Jae-Sue
Kim Kyu-Won
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Abstract
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Angiogenic activity of Aloe vera gel was investigated by in vitro assay. We obtained the most active fraction from dichloromethane extract of Aloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type I collagen gel and form capillary-like tube through in vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel through in vitro invasion assay. Furthermore, the effect on the MRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitory (PAl-1) mRNA was not changed.
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KEYWORD
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Angiogenesis, CPAR cells, In vitro angiogenesis assay, Urokinase-type plasminogen activator (u-PA), Matrix metalloproteinase (MMP)
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